| 產品名稱 |
GST-Cip1 |
| 商品貨號 |
B216128 |
| Designations |
GST-Cip1 |
| GenBank Number |
L25610
|
| Species |
Homo sapiens, human |
| Depositors |
SJ Elledge |
| Applications |
produces protein cyclin-dependent kinase inhibitor 1A (p21, Cip1) melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein) |
| Vector |
Construct size (kb): 6.0 DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pGEX-2TKcs
Intact vector size: 5.000
Type of vector: plasmid
Vector end: XhoI
Vector end: EcoRI
Cloning sites: NdeI NcoI XhoI BamHI EcoRI
Polylinker sites: NdeI NcoI XhoI BamHI EcoRI
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: tac
replicon: pMB1
Cross references: |
| Insert |
DNA: cDNA DESCRIPTION OF INSERT COMPONENT:
Genome: Homo sapiens
Gene symbol: CDKN1A
Other gene symbols: CIP1; WAF1*
Gene name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Contains complete coding sequence?: Y
Chromosome: 6; Localization: 6 p21.2
Tissue: B lymphocyte (EBV transformed)
Type of DNA: cDNA
Insert 5' end: XhoI
Insert 3' end: EcoRI
Insert size (kb): 1.03
Cross references: DNA Seq. Acc.: L25610 Insert lengths(kb): 1.029999971389771 Tissue: B lymphocyte (EBV transformed) Gene product: cyclin-dependent kinase inhibitor 1A (p21, Cip1)( melanoma differentiation associated protein 6, cyclin-dependent kinase inhibitor (p21), wild type p53 activated fragment 1 (Cdk-interacting protein)) [CIP1] Target Gene: cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
| Insert Size (kb) |
1.030 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Comments |
Restriction digests of the clone give the following sizes (kb): BglI--2.6, 1.5, 1.3, 0.55; EcoRI/XhoI--5.0, 1.1; KpnI--6.0; PstI/XbaI--3.4, 1.4, 1.15; PvuII--4.4, 1.5, 0.3. The insert contains the following restriction sites (approximate kb from the 5' end): BglI--0.15, 0.62; KpnI--0.94. Contains the complete coding sequence (nt 79-573 of the sequence record). Met-1 of the product is linked to GST through the linker sequence SHMPMARGHEGPEVSSLWSRSWARIRRGTEALRGGA. |
| References |
Harper JW, et al. The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases. Cell 75: 805-816, 1993. PubMed: 8242751
Stephen J Elledge, personal communication
|
