| Comments |
Restriction digests of the clone give the following sizes (kb): BamHI/XbaI--3.2, 2.2; NdeI--5.3; SspI/XhoI--2.2, 1.0, 0.66 (doublet), 0.55, 0.28. Insert contains the following restriction sites (approximate kb from the 5' end): EcoRV--0.76; HindIII--0.39, 1.82; NdeI--1.68; NcoI--0.52; PvuII--0.28, 0.85; XhoI--0.18. Digestion with XhoI and SspI released a 0.95 kb fragment containing the entire open reading frame. |
| References |
Saxena R, et al. The DAZ gene cluster on the human Y chromosome arose from an autosomal gene that was transposed, repeatedly amplified and pruned. Nat. Genet. 14: 292-299, 1996. PubMed: 8896558
David C Page, personal communication
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