Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Isolation
Macaca fascicularis, Malaysia, 1933
Product Format
frozen
Type Strain
no
Growth Conditions
Duration: in vivo, Macaca mulatta
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Subcultivation
Protocol: ATCCNO: 30037 SPEC: This strain is distributed as a frozen stabilate. See general instructions for thawing and storage of frozen material before proceeding. As soon as the strain arrives, remove the frozen ampule from the dry ice and transfer it directly to a 35C water bath. When it is completely thawed, aseptically remove the material with a syringe and inject the entire contents of the vial into an Aotus trivirgatus. This parasite can be maintained by serial passage of parasitized blood (by intravenous inoculation) into monkeys (Aotus trivirgatus).
Name of Depositor
K Powers
Chain of Custody
ATCC <<--K Powers<<--H.W. Mulligan/J.A. Sinton
Year of Origin
1933
References
Coatney GR, et al. Transmission of the M strain of Plasmodium cynomolgi to man. Am. J. Trop. Med. Hyg. 10: 673-678, 1961. PubMed: 13694174
Coatney GR. The primate malarias. Washington, DC: Government Printing Office; 1971.
Rec. Malar. Surv. India 3: 529-568, 1933.
Warren M, Garnham PC. Plasmodium cynomolgi: X-Irradiation and development of exo-erythrocytic schizonts in Macaco mulatta. Exp. Parasitol. 28: 551-556, 1970. PubMed: 5499672
