| Insert |
DNA: genomic Insert lengths(kb): 3.599999904632568 Tissue: 380 cell line Gene product: proviral integration, MCF [PIM] Alleles: A1, A2, B1, B2, C1, A1, A2, B1, B2, C1, C2, C2 |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--3.9, 3.05; HindIII--3.6, 2.5, 0.9; PstI--2.45, 1.5, 1.2, 1.1, 0.54; BamHI--5.2, 1.2, 0.48; SalI--7.0. Insert contains BamHI (2) and HindIII (2) sites. Includes part of intron 4, exon 5, intron 5, exon 6, and 3' flanking sequences. Cross-hybridizes to mouse Pim-1 gene under stringent conditions. Vector PyT is a pBR322 derivative with a deletion between EcoRII sites 1058 and 2500. Enzyme(s) not detecting polymorphism: EcoRI, HindIII. |
| References |
Zoghbi HY, et al. Deletion and linkage mapping of eight markers from the proximal short arm of chromosome 6. Genomics 6: 352-357, 1990. PubMed: 1968423
Nagarajan L, et al. Localization of the human pim oncogene (PIM) to a region of chromosome 6 involved in translocations in acute leukemias. Proc. Natl. Acad. Sci. USA 83: 2556-2560, 1986. PubMed: 3458216
Meeker TC, et al. Characterization of the human PIM-1 gene: a putative proto-oncogene coding for a tissue specific member of the protein kinase family. Oncogene Res. 1: 87-101, 1987. PubMed: 3329711
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