| 產(chǎn)品名稱 |
pACYC184 |
| 商品貨號(hào) |
B211873 |
| Designations |
pACYC184 |
| Depositors |
SN Cohen |
| Biosafety Level |
1 |
| Host |
Escherichia coli PM191 |
| Vector Information |
Vector size (kb): 4.244
Markers: cmlR, tetR
DNA Seq. Acc.: X06403
Type of vector: plasmid
Cloning sites: EcoRI HindIII BamHI SalI
Construction: p15A, pSC101, Tn9
Host range: Escherichia coli
Features):
marker(s): cmlR, tetR
replicon: p15A
Suggested host(s):
Escherichia coli chi-1776 (ATCC® 31244™)
Escherichia coli chi-1776 (ATCC® 31537™)
Escherichia coli chi-1776 (ATCC® 39234™)
Escherichia coli chi-1776 (ATCC® 53705™)
Escherichia coli C600 (ATCC® 23724™) |
| Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; EcoRI/BamHI--2.4, 1.9. Low copy number plasmid vector containing the replication system of miniplasmid p15A. |
| Media |
ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
|
| Growth Conditions |
Temperature: 37°C |
| References |
Chang AC, Cohen SN. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol. 134: 1141-1156, 1978. PubMed: 149110
Rose RE. The nucleotide sequence of pACYC184. Nucleic Acids Res. 16: 355, 1988. PubMed: 3340533
|
| Shipped |
frozen |
| Shipping Information |
Frozen glycerol stock of Escherichia coli containing the plasmid. |
