| 產品名稱 |
22D1 |
| 商品貨號 |
B210349 |
| Organism |
Cricetulus migratorius (B cell); Mus musculus (myeloma), hamster, Armenian (B cell); mouse (myeloma) |
| Cell Type |
hybridoma:lymphoblast B lymphocyte; somatic cell hybri |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is useful for FACS and immunocytochemistry assays. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays. |
| Comments |
Animals were immunized with mouse SIGN-R1 protein and their spleen cells were fused with mouse myeloma cells. SIGN-R1 is a C-type lectin. The antibody selectively binds to mouse SIGN-R1 but not to cells transfected with other lectins. The antibody is useful for FACS and immunocytochemistry assays. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) to 4 X 10(4) viable cells/ml. Interval: Subculture when cells reach a concentration of 8 X 10(5) cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density). |
| Cryopreservation |
Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype |
hamster, Armenian IgG |
| Population Doubling Time |
12 hours |
| Name of Depositor |
CG Park, RM Steinman |
| Year of Origin |
May, 2003 |
| References |
Kang YS, et al. The C-type lectin SIGN-R1 mediates uptake of the capsular polysaccharide of Streptococcus pneumoniae in the marginal zone of mouse spleen. Proc. Natl. Acad. Sci. USA 101: 215-220, 2004. PubMed: 14694198
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